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Image Search Results
Journal: Cell
Article Title: Optimal-transport analysis of single-cell gene expression identifies developmental trajectories in reprogramming
doi: 10.1016/j.cell.2019.01.006
Figure Lengend Snippet: (A) Log-likelihood ratio of obtaining iPSC vs non-iPSC fate on each day (x-axis) in 2i. Obox6+ cells in red. (B) Bright field and fluorescence images of iPSC colonies generated in 2i by overexpression of OKSM with either Zfp42 or Obox6 (or negative control). (C) Percentage of Oct4-EGFP+ colonies in 2i on day 16, for one of five experiments (Figure S6D). Error bars show standard deviation of three biological replicates. (D-F) Effect of varying concentration of GDF9 (red) vs control (grey) on (D) Oct4-EGFP+ colonies (error bars show standard deviation); (E) the strength of iPSC signature score in bulk RNA-Seq; and (F) cellular composition assayed by scRNA-seq. (G) Schematic of the reprogramming landscape in serum. Color indicates cell-set membership. Color of TFs indicates which cell set they regulate. Color of cytokine indicates the cell class to which they signal. See also Figure S6.
Article Snippet: To determine the effect of GDF9 on reprogramming, we plated secondary MEFs at a concentration of 5,000 cells per well of a 24-well plate and added either
Techniques: Fluorescence, Generated, Over Expression, Negative Control, Standard Deviation, Concentration Assay, Control, RNA Sequencing